Cellular Reprogramming for Immunotherapy

This volume brings together experimental and analytical methods on cellular reprogramming and immunotherapies. Chapters detail reprogramming immune cells, nonviral approaches, microRNA-based reprogramming, pharmacological control of immune cell states, plasmid electroporation, cell-state analysis and engineering, synthetic biology, functional genomics, scalable cell manufacturing, in vivo immune cell engineering, vector-mediated reprogramming, and the clinical and regulatory pathways required to translate emerging cell therapies from discovery to application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Cellular Reprogramming for Immunotherapy aims to serve as a practical resource for researchers working to understand immune cell identity and to develop the next generation of cellular immunotherapies.