Method Development in Analytical HPLC

Method Development in Analytical HPLC covers essential information for the process of developing an HPLC method of analysis, including foundational information related to HPLC, discussion on sample types, properties of analytes and matrices in the samples, sample preparation which is an important step in analytical methods development, guidance in adopting analytical methods from the literature, development of original methods with the selection of the adequate type of HPLC, of specific columns, mobile phase, and detection technique with emphasis on the use of mass spectrometry for detection, as well as optimization and validation of an analytical method. It also includes useful details on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, size exclusion, chiral etc. Method Development in Analytical HPLC includes information about green chemistry in analytical methods, computer assisted method development, and other modern aspects of the subject. Presents a systematic and logical presentation of foundational analytical HPLCGoes in depth on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, and size exclusionIncludes methods with emphasis on the use of mass spectrometry for detection