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A Bacterial Transgene for Catalase Protects Translation of D1 Protein

Name: A Bacterial Transgene for Catalase Protects Translation of D1 Protein
Brand: LAP Lambert Academic Publishing
SKU: 14447167
Price: 46.81 EUR
Availability: InStock
Author: Khaled AlTaweel
ISBN: 9783848487905

Khaled AlTaweel

Language

English

Book Paperback

Libristo code: 14447167

Publishers LAP Lambert Academic Publishing, November 2015

Abstract The pBI101-katE binary vector harboring katE gene derived from Escherichia coli and driven... Full description

Libristo code: 14447167

114 b

46.81 €

91.56 лв

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Abstract The pBI101-katE binary vector harboring katE gene derived from Escherichia coli and driven by the promoter of the Rubisco small subunit gene of tomato, rbcS3C, was isolated and molecular characterized then transformed into competent cells using the heat chock method. The binary vector was transformed into competent Agrobacterium tumefaciens cells by two ways; the first way via the tri-parental mating method, and the second one by the electroporation method. The pBI101-katE construct was introduced into a dwarf tomato via Agrobacterium tumefaciens-mediated transformation. And also the construct was introduced into a tobacco by biolistic bombardment.Putative transgenic plants were regenerated and further screened by using PCR. PCR analysis of T1 plants from the independent calli revealed the presence of katE and nptII genes.

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About the book

Full name A Bacterial Transgene for Catalase Protects Translation of D1 Protein

Author Khaled AlTaweel

Language

English

Binding Book - Paperback

Date of issue 2016

Number of pages 100

EAN 9783848487905

Libristo code 14447167

Publishers LAP Lambert Academic Publishing

Weight 165

Dimensions 150 x 220 x 6

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